Validating the growth increment periodicity in the otoliths of three small progenetic gobies

We determined the efficacy of marking otoliths three small-sized progenetic gobies to validate their increment periodicity. These small have high mortalities and rearing difficulties, making direct validation difficult. The were marked by immersing fish in a bath alizarin red S. fishes euthanatized number increments laid down after fluorescent mark counted compared with elapsed days. results validated daily periodicity Aphia minuta Pseudaphya ferreri. mortality hindered Crystallogobius linearis.